Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the mRNA expression of CTR1 (A), ATP7A (B), ATOX1 (C), ASCT2 (D) and PepT1 (E) genes in jejunal mucosa. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; ATP7A = ATPase copper transporting alpha; ATOX1 = Antioxidant 1 copper chaperone; ASCT2 = lanine-serine-cysteine transporter, type-2; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the mRNA Expression of CTR1 (A), ATP7A (B), ATP7B (C), ATOX1 (D), ASCT2 (E) and PepT1 (F) genes in liver. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; ATP7A = ATPase copper transporting alpha; ATOX1 = Antioxidant 1 copper chaperone; ASCT2 = lanine-serine-cysteine transporter, type-2; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Animal Nutrition

Article Title: Different copper sources and levels affect growth performance, copper content, carcass characteristics, intestinal microorganism and metabolism of finishing pigs

doi: 10.1016/j.aninu.2021.10.007

Figure Lengend Snippet: Effects of different copper sources and levels on the protein expression for CTR1(A), and PepT1(B) in jejunal mucosa. CuSO 4 = copper sulfate; Cu-Gly = copper glycinate; Cu-Pro = copper proteinate; CTR1 = high affinity copper uptake protein 1; PepT1 = peptide transporter 1. Data represent mean values ± standard error of the mean ( n = 6). Significant differences between processing treatments are represented by different lowercase letters ( P < 0.05).

Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.05% Tween-20 (TBST) and incubated overnight at 4 °C with the antibodies: rabbit anti-CTR1 (1:1,000, Genetex, GTX30642), rabbit anti-PepT1 (1:1,000, Bioss, bs-0689R) and rabbit anti-actin (1:1,000, Absin, abs132001).

Techniques: Expressing

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Primer sequences for real-time PCR amplification.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Real-time Polymerase Chain Reaction, Amplification, Sequencing

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 28 days of age. Values are means ± SE, ( n = 7/8). Lacking the same letters (a, b, and c) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; EAAT3, excitatory amino acid transporter 3; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The mRNA expression levels were calculated as the relative quantities (RQs) of the target gene mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 39 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a and b) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; EAAT3, excitatory amino acid transporter 3; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The mRNA expression levels were calculated as the relative quantities (RQs) of the target gene mRNA to the geometric mean of β-actin and GAPDH mRNA using the 2 −ΔΔCT method.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the protein expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 28 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a, b, and c) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6). The protein expression levels were calculated as the relative quantities (RQs) of the target gene protein to the β-tubulin protein.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Journal: Frontiers in Physiology

Article Title: Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers

doi: 10.3389/fphys.2022.952941

Figure Lengend Snippet: Effect of dietary Zn source on the protein expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 39 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a and b) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Qf = 51.6). The protein expression levels were calculated as the relative quantities (RQs) of the target gene protein to the β-tubulin protein.

Article Snippet: Western blotting analyses for rabbit anti-human ZnT1 (ab214356, Abcam, diluted 1:1,000), rabbit anti-mouse ZnT4 (BA3484, Boster, diluted 1:1,000), rabbit anti-human ZnT5 (25604-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT7 (13966-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human ZnT9 (BS62531, Bioworld, diluted 1:1,000), rabbit anti-human ZnT10 (ab229954, Abcam, diluted 1:1,000), rabbit anti-human ZIP3 (ab254868, Abcam, diluted 1:500), rabbit anti-human ZIP5 (ab105194, Abcam, diluted 1:1,000), rabbit anti-human B 0 AT1 (ab180516, Abcam, diluted 1:1,000), rabbit anti-human LAT1 (DF8065, Affinity Biosciences, diluted 1:500), rabbit anti-human y + LAT2 (13823-1-AP, Proteintech, diluted 1:1,000), rabbit anti-human rBAT (DF7379, Affinity Biosciences, diluted 1:1,000) and rabbit anti-human PepT1 (A03672-1, Boster, diluted 1:500) were carried out according to the recommended protocols provided by the manufacturers.

Techniques: Expressing

Journal: Antioxidants

Article Title: The Cytotoxicity of Doxorubicin Can Be Accelerated by a Combination of Hyperthermia and 5-Aminolevulinic Acid

doi: 10.3390/antiox10101531

Figure Lengend Snippet: Western blot analysis of each transporter expression. SLC22A16 ( A , B ), ABCG2 ( C , D ) and SLC15A1 ( E , F ) were normalized to β-actin expression. Data are expressed as the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01. Student’s t -test.

Article Snippet: Antirabbit SLC22A16 (cat. no. LS-C176922) (LSBio, Seattle, WA, USA), ABCG2 (cat. no. 42078) (Cell Signaling Technology Japan K.K., Tokyo, Japan), or SLC15A1 (cat. no. bs-10588R) (Bioss, Boston, MA, USA) antibodies (1:1000) were added to the Can Get Signal ® Immunoreaction Enhancer Solution 1 (Toyobo Co. Ltd., Osaka, Japan).

Techniques: Western Blot, Expressing